Fig. 1. Expression of APJ receptors in mpkCCD cells. Representative immunoblot of 4 independent experiments showing APJ receptor in both mpkCCD cells and rat brain tissue. Protein extracts from HEK293 cells were used as a negative control. In a and b, 5 and 10 µg of protein were loaded, respectively. Total protein levels were normalized to GAPDH levels, used as a control.